Figure 5 | Scientific Reports

Figure 5

From: Increasing endogenous activity of NMDARs on GABAergic neurons increases inhibition, alters sensory processing and prevents noise-induced tinnitus

Figure 5

M-8324 infusion prior to noise exposure reduced PV-neuron loss and increased inhibitory synaptic transmission in AI in the noise-exposure (NE) mice. (A) Representative images of staining of PV-neurons in AI of control, NE + veh and NE + M-8324 group. Scale bar, 100 µm. (B) Density of PV-neurons in AI layer 2/3 in. N = 30 sections/5 mice for Ctrl group, N = 22 sections/5 mice for NE + veh group, N = 15 sections/4 mice for NE + M-8324 group (C) Sample traces of spontaneous inhibitory postsynaptic current (sIPSC) from AI excitatory neurons in NE + veh group and NE + M-8324 group. Scale bars, 40 pA/500 ms. (D) A significant reduction in the decay time of sIPSCs in NE group. (E) Amplitude of sIPSC in different among groups. (F) Charge of sIPSC in different among groups. (G) A significant increase in the frequency of sIPSC was seen in NE + M-8324 group. N = 17 cells/4 mice for Ctrl group, N = 23 cells/4 mcie for NE + veh, N = 10 cells/4 mice for NE + M-8324. Data are presented as the mean ± SEM.

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