Figure 3 | Scientific Reports

Figure 3

From: Robust phenotypic maintenance of limb cells during heterogeneous culture in a physiologically relevant polymeric-based constructed graft system

Figure 3

In vitro evaluations of the physiological functions and phenotypic characteristics in each region of the CGS during the heterogeneous culture. (A) Representative live/dead staining images for regions A, B, and C at 3 and 7 days (n = 3, and 4–6 random fields/sample). (B) Proliferation of SFs, STFs, and OBs in regions A, B and C, respectively at 3 and 7 days (n = 3, ANOVA and post hoc Tukey test, *P < 0.05, **P < 0.01, ***P < 0.001). (C) Immunofluorescent staining of regions A, B, and C for VIM, SCXA, and ALP at 3 and 7 days, respectively. (D) Quantifications of VIM, SCXA and ALP expression from regions A, B and C at 3 and 7 days, respectively (n = 3, and 4–6 random images/sample, ANOVA and post hoc Tukey test *P < 0.05, **P < 0.01). (E) Immunofluorescent staining of regions A and B for ALP at 3 and 7 days, respectively. (F) Quantifications of ALP expression from regions A and B at 3 and 7 days, respectively (n = 3, and 4–6 random images/sample, ANOVA and post hoc Tukey test). (G) Representative F-actin staining images for regions A, B, and C at 3 and 7 days (n = 3, and 4–6 random fields/sample).

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