Figure 1
From: MAF1 is a chronic repressor of RNA polymerase III transcription in the mouse
Altered pol III occupancy in livers from fasted and refed WT mice. (A) Average pol III occupancy scores, Log2(IP/input), for two replicate ChIP-seq experiments performed with pooled liver samples from three wild-type mice fasted for 8 h or fasted for 8 h and refed for 4 h. Each dot corresponds to a pol III locus and is colored according to its score quantile in the WT_Refed sample. (B) MvA plot showing the average occupancy scores in refed and fasted conditions (x-axis) and the log2 fold change (fasted/refed) between these conditions (y-axis). Red dots, loci with significant changes by limma analysis (adj. p value < 0.01); black dots, pol III loci with scores above the cut-off in at least one sample that were not significant by limma analysis (adj. p value ≥ 0.01), grey dots, pol III loci with scores below the cut-off in both samples. See SI Table S2 for corresponding scores and limma analysis. (C) Color mapping of pol III occupancy relationships between nutritional and proliferative signaling responses. The fasting/refeeding data in panel B are colored based on the fold change in occupancy scores before and 36 h after partial hepatectomy (PH)18. Black dots, no occupancy change upon feeding/fasting or PH; green dots, reciprocal changes in pol III occupancy upon feeding/fasting and PH; red dots, genes with higher sensitivity to PH than to feeding; blue and magenta dots, genes with higher sensitivity to feeding than to PH.
