Figure 3
From: MAF1 is a chronic repressor of RNA polymerase III transcription in the mouse
Maf1−/− mice have higher levels of pre-tRNAs in refed and fasted conditions. (A) Northern blot analysis of liver and WAT mature-length and precursor tRNAs in 4 h refed conditions. The intensity of each band was quantified and the mean values and standard errors (SEM) for the WT and Maf1−/− independent biological replicates are indicated below each panel. Small nucleolar RNA U3 was used as a loading control. Note that all blots represent contiguous panels from the same gels. The images have been cropped at the top and the bottom to frame the relevant region for presentation. (B) Northern blot analyses of pre-tRNAIle-TAT (locus n-Ti16, see SI Table S1) in the indicated tissues from 4 h refed or 16 h fasted WT and Maf1−/− mice. The fold change in pre-tRNA normalized to U3 snRNA was expressed relative to the mean WT refed value for each tissue. WT and Maf1−/− are represented by blue and orange columns, respectively, with solid colors reporting refed values and solid colors with cross-hatching reporting fasted values. Northern blot analyses of normalized pre-tRNAs (C) and mature tRNAs (D) in refed liver samples (WT n = 3, KO n = 4) were expressed relative to the mean value for WT (± SEM). For panel (C), p values (Student’s standard t test) are: pre-tRNALeu 0.07; pre-tRNAIle 0.007; pre-tRNATyr 0.052; pre-tRNASer 0.03 and pre-tRNAHis 0.025 and are indicated as *< 0.05 and **< 0.01. Abbreviated locus information is annotated underneath each pre-tRNA, as listed in SI Table S1, column C.
