Figure 1

Frequency of aneuploid HSC cells in the simulated post-transplantation bone marrow. (A) Frequency of aneuploid cells observed in peripheral blood of recipient mice after receiving transplanted bone marrow from BUBR1^H/H (hypomorphic) mice; data are from Pfau et al.¹⁵, except for data at days 350 and 364 which was collected following the protocol in Pfau et al.¹⁵; FL transplanted fetal liver cells, BM transplanted bone marrow cells; see Supplements section Aneuploidy counts for a summary of data. (B) Simulated aneuploidy dynamics with varying aneuploidy generation rate per cell division (numbers color-matched to respective data lines; statistics in Supplementary Fig. S2). (C) Simulated aneuploidy dynamics with a range of cell fitness cost induced by aneuploidy (statistics in Supplementary Fig. S3). (D) Dynamics of HSC population increase post transplantation over time (color-matched numbers represent growth coefficients which determined the shape of the population size growth). (E) Simulated aneuploidy dynamics under various cell population expansion regimens (numbers color matched as in (D); statistics in Supplementary Fig. S4). (F) Simulated aneuploidy frequency at stable cell division rate of 1 in 20 days and various extent of cell population size expansion (color-matched numbers indicate initial and final population size in # of cells; statistics in Supplementary Fig. S5; a higher range of pool sizes is also shown in Supplementary Fig. S6). (G) Simulated aneuploidy frequency at a stable cell division rate of 1 in 20 days and different stable cell population sizes (color-matched numbers indicate population size in # of cells; statistics in Supplementary Fig. S7). (H) Simulated aneuploidy frequency at a stable population size of 10,000 cells and varying stable cell division rates (color-matched numbers indicate the average interval in days between successive cell divisions; statistics in Supplementary Fig. S8). (I) Simulated aneuploidy frequency under population expansion from 1,000 to 10,000 cells and varying stable cell division rates (color-matched numbers as in (H); statistics in Supplementary Fig. S9).