Figure 1 | Scientific Reports

Figure 1

From: Pro-angiogenic scaffold-free Bio three-dimensional conduit developed from human induced pluripotent stem cell-derived mesenchymal stem cells promotes peripheral nerve regeneration

Figure 1

Generation of the Bio 3D conduit from xeno-free iMSCs. (A) Experimental design for the Bio 3D conduit. iMSCs are cultured on a temperature-responsive plate to generate a cell sheet. The cell sheets are then transferred to a low attachment plate to generate clumps of the iMSC/ECM complex, C-iMSCs. C-iMSCs are arranged into a needle array to generate the Bio 3D conduits and transplanted into a model rat. Eight weeks later, the recovery of the nerve injury was analyzed. (B) Images of cell sheets, C-iMSCs, pre-designed computer models, and Bio 3D structures in the Bio 3D conduit-generating step. After 1 week of perfusion, C-iMSCs are fused together and transferred to the catheter. Because the structures are shortened on the catheter, two conduits are fused together to achieve sufficient length. Scale bars: Cell sheet and C-iMSC, 500 µm; 3D structure and Bio-3D conduit, 2 mm. (C) Section images of C-iMSCs and the Bio 3D conduit. Staining with H&E (left) or anti-hVimentin (green, middle) and anti-Collagen1 (Col1) (red, right). Nuclei were stained with DAPI (blue). Scale bars, 500 µm.

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