Figure 2 | Scientific Reports

Figure 2

From: Pro-angiogenic scaffold-free Bio three-dimensional conduit developed from human induced pluripotent stem cell-derived mesenchymal stem cells promotes peripheral nerve regeneration

Figure 2

Cells composing the Bio 3D conduit maintained MSC properties. (A) Global gene expression profile. PCA analysis indicates no obvious change in the cell character in each Bio 3D conduit-generating step. (B) Heatmap illustrating the expression of pluripotent stem cell (PSC), neural crest cell (NCC), and mesenchymal stem cell (MSC) markers for cells in each Bio 3D conduit-generating step. (C) The mRNA expression of MSC marker genes was confirmed by RT-qPCR. MSC marker genes were expressed normally, indicating the MSC character was maintained. *P < 0.05, **P < 0.01 by Dunnett’s multiple comparisons t test compared with iPSCs. (D) iMSCs expressed surface markers for MSCs (CD44, CD90, CD90 and CD105), and were negative for CD45 and HLA-DR.

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