Table 1 Primers of candidate reference genes.
Symbol | Name | Function | Assay ID (Bio-Rad) | Amplicon length (bp) | Product efficiency |
|---|---|---|---|---|---|
ACTB | actin, beta | Cytoskeletal structural protein | qHsaCED0036269 | 62 | 2.03 |
B2M | beta-2-microglobulin | Beta chain of MHC class I | qHsaCID0015347 | 123 | 1.98 |
G6PD | glucose-6-phosphate dehydrogenase | Maintain the level of NADPH | qHsaCED0001353 | 137 | 2.05 |
GAPDH | glyceraldehyde-3-phosphate dehydrogenase | Glycolysis and gluconeogenesis | qHsaCED0038674 | 117 | 1.97 |
GUSB | glucuronidase, beta | Carbohydrate metabolic process | qHsaCID0011706 | 79 | 2.03 |
HMBS | hydroxymethylbilane synthase | Heme biosynthetic process | qHsaCID0038839 | 137 | 1.98 |
HPRT1 | hypoxanthine phosphoribosyltransferase 1 | Purine salvage pathway | qHsaCID0016375 | 90 | 2.05 |
PGK1 | phosphoglycerate kinase 1 | Glycolysis and gluconeogenesis | qHsaCED0003721 | 74 | 1.81 |
RPL13A | ribosomal protein L13a | Component of the 60S ribosomal subunit | qHsaCED0020417 | 118 | 1.99 |
RPLP0 | ribosomal protein, large, P0 | Component of the 60S ribosomal subunit | qHsaCED0038653 | 63 | 1.95 |
RPS18 | ribosomal protein S18 | Component of the 40S ribosomal subunit | qHsaCED0037454 | 67 | 1.94 |
TBP | TATA box binding protein | Ttranscription factor binding | qHsaCID0007122 | 120 | 2.01 |
TFRC | transferrin receptor | Transferrin transport | qHsaCID0022106 | 102 | 1.96 |
YWHAZ | 14-3-3 protein, zeta polypeptide | Major regulator of apoptotic pathways | qHsaCID0013897 | 175 | 1.96 |
