Table 2 Expression stability and ranking of reference genes, evaluated based on ΔCt method, BestKeeper, NormFinder, and geNorm.

From: RT-qPCR analyses on the osteogenic differentiation from human iPS cells: an investigation of reference genes

Reference gene

ΔCt

BestKeeper

NormFinder

geNorm

Comprehensive ranking

Mean SD

Rank

SD

Rank

Coeff. of corr

Rank

General rank

Stability value

Rank

M value

Rank

Geometric mean of ranks

General rank

ACTB

0.739

9

0.650

9

0.777*

7

10

0.678

13

0.591

10

10.400

11

B2M

1.185

14

0.697

11

0.001

14

14

0.891

14

0.751

14

14.000

14

G6PD

0.838

13

0.818

13

0.816*

6

12

0.500

12

0.674

13

12.490

13

GAPDH

0.654

5

0.337

3

0.610*

11

7

0.168

6

0.404

4

5.384

5

GUSB

0.651

4

0.110

1

0.596*

12

2

0.127

3

0.386

3

2.913

4

HMBS

0.788

11

0.827

14

0.876*

4

9

0.162

5

0.620

11

8.590

9

HPRT1

0.805

12

0.635

8

0.642*

10

13

0.290

9

0.647

12

11.393

12

PGK1

0.699

7

0.762

12

0.930*

2

5

0.199

7

0.476

6

6.192

6

RPL13A

0.698

6

0.544

6

0.751*

8

8

0.225

8

0.535

8

7.445

7

RPLP0

0.568

2

0.342

4

0.869*

5

4

0.128

4

0.339

1

2.378

3

RPS18

0.746

10

0.307

2

0.106

13

6

0.323

10

0.512

7

8.050

8

TBP

0.560

1

0.416

5

0.906*

3

3

0.068

1

0.339

1

1.316

1

TFRC

0.634

3

0.651

10

0.936*

1

1

0.068

1

0.443

5

1.968

2

YWHAZ

0.718

8

0.567

7

0.721*

9

10

0.440

11

0.564

9

9.434

10

  1. *P < 0.05.
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