Table 3 Primers of target genes.
Assay | Symbol | Name | Function | Assay ID |
|---|---|---|---|---|
PrimePCR SYBR Green Assay (Bio-Rad) | TBP | TATA box binding protein | Transcription factor binding | qHsaCID0007122 |
POU5F1 | POU class 5 homeobox 1 | Marker for undifferentiated cells | qHsaCED0038334 | |
NANOG | Nanog homeobox | Marker for undifferentiated cells | qHsaCED0043394 | |
ALP | Alkaline phosphatase, liver/bone/kidney | Matrix mineralization | qHsaCED0045991 | |
OC | Osteocalcin | Calcium-binding protein hormone | qHsaCED0038437 | |
DMP1 | Dentin matrix acidic phosphoprotein 1 | Extracellular matrix mineralization | qHsaCID0015301 | |
SOST | Sclerostin | Produced primarily by the osteocyte | qHsaCED0046506 | |
Taqman Gene Expression Assay (Applied Biosystems) | RUNX2 | Runt related transcription factor 2 | Key transcription factor for osteoblast differentiation | Hs00231692_m1 |
SP7 | Sp7 transcription factor | Transcription factor for osteoblast differentiation | Hs01866874_s1 | |
COL1A1 | Collagen type I alpha 1 | Fibrillar collagen | Hs00164004_m1 |
