Figure 3 | Scientific Reports

Figure 3

From: High-resolution and differential analysis of rat microglial markers in traumatic brain injury: conventional flow cytometric and bioinformatics analysis

Figure 3

Microglia Polarization at 24 h after Injury (Traditional Analysis). Cells gated under CD45+CD11b/c+P2y12+ were further characterized according to M1 associated markers CD32 + or CD86 + (panel A) and M2 associated markers CD200R + ,RT1B + , or CD163 + (panel B). Mean fluorescence intensity (MFI) was measured in both ipsilateral and contralateral hemispheres of sham and CCI brains 24 h after injury. We conclude that these microglia subpopulations express significantly higher CD32 and CD163, while also expressing significantly lower CD86 and RT1B. Data represent mean values ± SD (n = 3). Statistical analysis performed by Two-Way ANOVA and Uncorrected Fisher’s Least Significant Difference (LSD) test. (C) Mann–Whitney U Test to evaluate the differences between the ipsilateral/contralateral event measurements and the corresponding sham measurements, adjusting for Benjamini–Hochberg false discovery rate (FDR) of 0.05.

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