Table 2 Primer sequences for RT-PCR and real-time PCR.
Gene | Primer | Oligonucleotide sequence (5′–3′) |
|---|---|---|
GAPDH | F | 5′-CAATGAATACGGCTACAGCAAC-3′ |
R | 5′-AGGGAGATGCTCAGTGTTGG-3′ | |
iNOS | F | 5′-CCCTTCCGAAGTTTCTGGCAGCAGC-3′ |
R | 5′-GGCTGTCAGAGCCTCGTGGCTTTGG-3′ | |
COX-2 | F | 5′-TCTCAGCACCCACCCGCTCA-3′ |
R | 5′-GCCCCGTAGACCCTGCTCGA-3′ | |
IL-1β | F | 5′-CAGGGTGGGTGTGCCGTCTTTC-3′ |
R | 5′-TGCTTCCAAACCTTTGACCTGGGC-3′ | |
TNF-ɑ | F | 5′-TTGACCTCAGCGCTGAGTTG-3′ |
R | 5′-CCTGTAGCCCACGTCGTAGC-3′ | |
IL-6 | F | 5′-GTACTCCAGAAGACCAGAGG-3′ |
R | 5′-TGCTGGTGACAACCACGGCC-3′ | |
IL-17 | F | 5′-TCTCATCCAGCAAGAGATCC-3′ |
R | 5′-AGTTTGGGACCCCTTTACAC-3′ | |
CCR3 | F | 5′-CCCGAACTGTGACTTTGCT-3′ |
R | 5′-CCTCTGGATAGCGAGGACTG-3′ | |
MUC5AC | F | 5′-AGAATATCTTTCAGGACCCCTGCT-3′ |
R | 5′-ACACCAGTGCTGAGCATACTTTT-3′ |
