Figure 1
From: The HERC1 ubiquitin ligase regulates presynaptic membrane dynamics of central synapses
Electron microscopy microphotographs illustrating the ImageJ counts of the number of synaptic vesicles in control (A) and tbl (B) hippocampal cultures. The active zone is consistently shorter in tbl than in control presynaptic boutons (D; *p = 0.0246). Quantitative analyses demonstrate that tbl synapses possess less synaptic vesicles than control ones (C; *p = 0.0391), and fewer docked synaptic vesicles in both absolute (F AZ, *p = 0.0470) and normalized values (F; 500 nm AZ, *p = 0.0265). However, no significant statistic differences are found in the synaptic vesicles diameter (E; ∅, p = 0.1452), the intervesicular distance (E, iV distance, p = 0.2184), in the number of tethered vesicles (G; AZ, p = 0.2011; 500 nm AZ, p = 0.0834), and in the number of vesicles respecting their distance from the active zone (H; < 75 nm, p = 0.0853; 75–150 nm, p = 0.4834; 150–225 nm, p = 0.4342; 225–300 nm, p = 0.2318). Bars = 200 nm.
