Figure 3
Stimulation during neurogenesis affects key morphological parameters of network formation. a. Representative phase contrast images of neurite extension along the periphery of embryoid bodies between non-stimulated (NS) and stimulated during neurogenesis (S) samples (scale bar: 50 µm). b. Bar graphs representing the average number of neurites protruding from the periphery of embryoid body normalized by the perimeter of the embryoid body at a given time after seeding. Each point signifies the number of extending neurites normalized by the perimeter of an individual embryoid body (n = 20; error bar represents SEM, *p < 0.05, ANOVA with Tukey post-hoc test). c. Representative fluorescence images of synaptic puncta stained against SY38 at D11 along a neurite. Arrow denote presynaptic puncta. (scale bar: 5 µm). d. Bar graphs representing the average number of presynaptic puncta along the length of neurites for D11. Each point corresponds to the average number of synaptic puncta along a neurite normalized the length of the neurite per field of view (n = 10; error bar represents SEM, *p < 0.05, ANOVA with Tukey post-hoc test). e. Bar graphs representing the average number of presynaptic puncta per unit area for D11-D15. Each point corresponds to the average number of synaptic puncta per unit area in an individual field of view (n = 10; error bar represents SEM, *p < 0.05, ANOVA with Tukey post-hoc test).
