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Figure 1

From: 11-Deoxycortisol controls hydromineral balance in the most basal osmoregulating vertebrate, sea lamprey (Petromyzon marinus)

Figure 1

Lamprey corticosteroid biosynthesis and characterization of the lamprey CR. (A) Corticosteroid biosynthetic pathway; dotted box represents the pathway components present in lamprey (cyp11b genes are absent in lamprey). Corticosteroid abbreviations: PREG, pregnenolone; 17P5, 17-hydroxypregnenolone; P, progesterone; 17OHP, 17-hydroxyprogesterone; DOC, deoxycorticosterone; S, 11-deoxycortisol; B, corticosterone; A, aldosterone. Chemical structures were drawn using MarvinSketch Version 20.13 (ChemAxon; https://www.chemaxon.com). (B) Representative receptor binding curves for total binding (BT), nonspecific binding (BNS), and specific binding (Bs = BT − BNS). (C) Representative specific binding saturation curve showing calculated values for corticosteroid receptor abundance (Bmax) and equilibrium dissociation constant (Kd); insert: Rosenthal plot of saturation curve (B = bound, F = free). (D) Relative binding affinity for endogenous (S and DOC) and later-evolved (F and A) corticosteroids (n = 3; values represent mean ± SEM); IC50 (half-maximal inhibition) values: IC50(S) = 15.7 nM; IC50(DOC) = 89.1 nM (unpaired t test: P < 0.001; n = 3).

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