Figure 4 | Scientific Reports

Figure 4

From: 11-Deoxycortisol controls hydromineral balance in the most basal osmoregulating vertebrate, sea lamprey (Petromyzon marinus)

Figure 4

Effects of in vivo S treatment on lamprey osmoregulation after SW exposure. Mid-metamorphic sea lamprey were administered S or a vehicle control (Veh), then exposed 12 days post-injection to 25‰ SW for 24 h. (A) Plasma [Cl−], (B) Gill NKA activity. (C,D) gill mRNA abundance of nka and nkcc1. (E,F) Representative bands and quantification of gill NKA and NKCC1 protein abundance. T0 = uninjected FW control sampled at time of S administration. Dose of S is stated in parenthesis (μg g−1 body weight). Values represent mean ± SEM and data points with different letters are significantly different (n = 8–10; one-way ANOVA (A–F: P < 0.001); Tukey’s post hoc).

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