Figure 4 | Scientific Reports

Figure 4

From: MCD diet-induced steatohepatitis generates a diurnal rhythm of associated biomarkers and worsens liver injury in Klf10 deficient mice

Figure 4

Klf10 deficiency does not impact the development of hepatic steatosis and inflammation during steatohepatitis in mice. WT and Klf10 -/- male mice were fed on a methionine and choline deficient diet (MCDD) for 4 weeks and sacrificed at ZT 3. (A) Representative images, showing the presence of lipid droplets and inflammatory foci (black arrows), from H&E stained liver sections of WT and Klf10-/- upon MCDD. (B) Quantification of hepatic steatosis and inflammatory foci from H&E stained liver sections (n = 8–11 mice/group). (C) Number of hepatic granulocytes and monocytes assessed by flow cytometry on hepatic non parenchymal cells stained for CD45, Ly6C and Ly6G. Data represents the number of cells/g of tissue (n = 5–6 mice/group). (D) Hepatic expression of Tnfα and Ccl2 (n = 8–12 mice/group). All data are expressed as mean ± SEM. The mRNA levels are normalized to B2m and expressed relative the CD ZT3 level (n = 12). Statistically significance was analyzed using the Mann–Whitney test vs control group WT CD, * p < 0.05, ** p < 0.01, **** p < 0.0001.

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