Figure 1

Targeted DIA-LCMS2/Skyline protein quantitation of glutamine synthetase protein (GS, XP_003444352.1) in cells grown in four different medium conditions: isosmotic (315 mOsmol/kg), hyperosmotic (650 mOsmol/kg), isosmotic + 10 µM actinomycin D, hyperosmotic + 10 µM actinomycin D. Data for one of the four quantified peptides, QQYMSLPQGEK, is shown. Each treatment consisted of five biological replicates (from left to right).