Table 1 Experimental conditions and physiological observations for growth on αKG as sole-carbon source and co-mixture with glucose in batch and chemostat conditions. More details on the batch results can be found in Fig. 1 and Supplementary Figure S2.
Growth mode | C-Source | pH | Growth? | Observations |
|---|---|---|---|---|
Batch | 2 g/L αKG | 2.5 | Yes | Growth is observed using αKG between pH 2.5 to 5. Both H2αKG and HαKG must be transported to explain obtained growth results |
3 | Yes | |||
4 | Yes | |||
5 | Yes | |||
6 | No | |||
2.5 g/L αKG | 4 | Yes | A growth rate of 0.087 ± 0.002 h−1. No decrease of growth rate for different concentrations of αKG | |
5 g/L αKG | Yes | |||
10 g/L αKG | Yes | |||
20 g/L Glucose 2.8 g/L αKG | 4 | Yes | Maximum growth rate using glucose and αKG is µ = 0.40 h−1. αKG is not consumed while the glucose concentration is high, nor does αKG inhibit growth | |
Chemostat | 10 g/L αKG | 3 | Yes | Residual αKG concentrations range from 30.1 to 37.9 mmol/L. The αKG uptake rate and residual αKG concentration were similar for the three different pH conditions |
4 | Yes | |||
5 | Yes | |||
6 | No | |||
20 g/L Glucose 2.8 g/L αKG | 4 | Yes | Both residual αKG (0.98 ± 0.28 mmol/L) and glucose (0.34 ± 0.01 mmol/L) are low, indicating that under continuous conditions co-consumption does also occur |
