Table 3 Steady state intracellular metabolite concentrations (μmol/gDW) for different cultivation conditions: αKG as sole carbon source, αKG and glucose co-feeding, and glucose only. PEP, Pyr, Succ and Glu were not measured for αKG only cultivation.
Condition | G6P | Pyr | Cit | iCit | αKG | Succ | Fum | Mal | Glu |
|---|---|---|---|---|---|---|---|---|---|
αKG | 1.14 ± 0.02 | – | 3.34 ± 0.09 | 0.11 ± 0.00 | 22.21 ± 7.70 | – | 4.16 ± 0.24 | 15.50 ± 1.02 | – |
αKG and glucose | 8.37 ± 0.20 | 0.33 ± 0.08 | 7.89 ± 0.39 | 0.17 ± 0.02 | 2.81 ± 0.35 | 1.49 ± 0.16 | 0.87 ± 0.10 | 4.00 ± 0.05 | 116.26 |
Glucose (taken from32) | 5.42 ± 0.15 | 2.20 ± 0.02 | 6.96 ± 0.15 | 0.40 ± 0.02 | 1.82 ± 0.04 | 0.96 ± 0.01 | 0.67 ± 0.01 | 3.01 ± 0.05 | 104.24 ± 1.51 |
