Figure 4
Influence of the linkage of the original five copies of 16S rDNA on the dPCR measurement data following a DNA extraction in view of the dPCR principle. 9 wells of a dPCR chip are presented representing 18,000 wells. L. paracasei cell originally has five copies of 16S rDNA coded on one chromosomal DNA. Upper-left image of micro-tube indicates typically used real-time PCR tube in which the dPCR master mix (direct master mix) containing template DNA (presented with open circular of â—‹ in its tube) was added. (a) Five copies of 16S rDNA coded on the same chromosomal DNA molecule. The five copies of 16S rDNA becomes dispersed to one well, which leads to a positive well, resultantly, measured as one copy. A magnified well highlighted with light-green colour contains five copies of 16S rDNA linked. A 8 wells with normal size of open circular indicates no targeted 16S rDNA copies are contained. (b) Five copies of 16S rDNA coded on the different relevant chromosomal DNA fragments. When the five copies of 16S rDNA are coded on the different five relevant chromosomal DNA fragments due to heat stress, the absolute dPCR determined it as five copies. Wells that contain the 16S rDNA copy at one copy are presented as closed circulars with light-green colour, and open circulars present wells that contain no 16S rDNA copies.
