Figure 8

Correlation of reporter driven bioluminescence and native transcript for bosR and dbpBA. To validate the bioluminescent reporters encoded on a multicopy shuttle vector accurately reflects native transcription a correlation of radiance and copies of the gene of interest within a specific tissue was performed. Total transcript copies of bosR from the skin flank (A) and heart (B) from 10 and 21 dpi was correlated with the representative radiance value. Additionally, dbpBA copies from the skin flank (C) and heart (D) from 10 and 21 dpi was correlated with appropriate radiance.