Figure 3
From: Adipocytes protect fibroblasts from radiation-induced damage by adiponectin secretion
A protein of > 50 kDa is responsible for enhanced fibroblasts viability following irradiation. Size exclusion columns were used to concentrate proteins either at > 3 kDa or > 50 kDa. We also tested the ultrafiltrates that contain protein < 3 kDa and < 50 kDa in normal concentrations. The normal growth media (NGM) and ultrafiltrates had significantly reduced viability 5 days after 5 Gy radiation treatment. The > 3 kDa concentrate provided some enhancement to viability but was still significantly reduced, whereas the > 50 kDa concentrate protected fully from radiation-induced cell death. Statistical significance was determined using a 1-way ANOVA with post hoc Tukey’s test for multiple comparisons (*) indicates p < 0.05 and (**) indicates p < 0.001 as compared to all sham irradiated conditions. n = 3 for all experimental conditions.
