Figure 4
From: Adipocytes protect fibroblasts from radiation-induced damage by adiponectin secretion
Adiponectin is a secreted factor present in conditioned media and exogenous addition of recombinant adiponectin protects fibroblasts from radiation-induced cell death. (A) Normal growth media, fibroblast conditioned media, and adipocyte conditioned media were assayed with an ELISA for mouse adiponectin. Adipocyte media contained significantly more (*p = 0.001) adiponectin than the other medias tested. (B) Recombinant mouse adiponectin equivalent to concentrations found in adipocyte conditioned media, 0.3 ng/mL, was added to normal growth media prior to 5 Gy irradiation. Cells were assessed for viability 5 days later using trypan blue. (*) indicates significantly less viability than all other groups (p < 0.0001). All statistical significance was determined by a 1-way ANOVA followed by post hoc Tukey’s test for multiple comparisons, n = 3 for all experiments.
