Figure 5
From: Adipocytes protect fibroblasts from radiation-induced damage by adiponectin secretion
Adipocyte conditioned media and exogenous adiponectin protect fibroblasts from radiation-induced activation. Fibroblasts were irradiated with 2 Gy in the presence of either fibroblast conditioned media, adipocyte conditioned media, or exogenous adiponectin. Two days later the cells were embedded in rat tail collagen discs and observed for their ability to contract extracellular matrix. (A) Five hours after embedding fibroblasts in collagen, the discs were removed from the incubator and imaged. (B) ImageJ was used to measure the total area of the contracted discs. For each biological replicate, three technical replicates were performed. Statistical analysis was assessed by a 1-way ANOVA followed by post hoc Tukey’s test for multiple comparisons. (*) indicates significant increase (p < 0.0001) as compared to any sham irradiated control. All experiments were completed in triplicate with three technical replicates each.
