Figure 6
From: Adipocytes protect fibroblasts from radiation-induced damage by adiponectin secretion
Adipocyte conditioned media and exogenous adiponectin protect fibroblasts from radiation-induced senescence. Fibroblasts were irradiated with 3 Gy in the presence of either normal growth media, fibroblast conditioned media, adipocyte conditioned media, or exogenous adiponectin. Three days later the cultures were assayed for β-galactosidase activity. (A) Representative images of cells stained for β-galactosidase activity, images were inverted to aid in visualizing positively stained cells (examples indicated by white arrows), which appear bright pink. (B) Graphical representation of quantified images as percent positively staining cells. Statistical analysis was determined by a 1-way ANOVA followed by post hoc Tukey’s test for multiple comparisons, n = 3 for all conditions. (*) indicates significant increase (p < 0.0001) as compared to any sham irradiated condition. (#) indicates significantly less senescence (p < 0.001) as compared to either irradiated fibroblasts in normal growth media or fibroblast conditioned media.
