Figure 7
From: Adipocytes protect fibroblasts from radiation-induced damage by adiponectin secretion
Adiponectin alone can reduce cancer cell growth and does not protect cancer cells from radiation damage. (A, B) Prostate cancer cells, PC3, and (C, D) colorectal cancer cells, HT29, were treated with PBS or 0.3 ng/mL of human recombinant adiponectin then either sham irradiated or treated with 3 Gy X-rays. Cells were immediately detached and seeded at low density for 10 days then fixed, stained, and colonies enumerated to determine clonogenic survival, quantified in (A, C). Representative images of stained colonies are displayed in (B, D). For each biological replicate (n = 3), three technical replicates were performed. Statistical analysis was performed with a 1-way ANOVA followed by post hoc Tukey’s test for multiple comparisons. For both cell lines adiponectin showed statistically significant differences between paired sham and irradiated treatments where (*) indicates p < 0.001 and (#) indicates p < 0.05.
