Figure 1 | Scientific Reports

Figure 1

From: Evaluating the quantity, quality and size distribution of cell-free DNA by multiplex droplet digital PCR

Figure 1

A multiplex ddPCR assay to QC cfDNA samples. Our assay permits discriminating between OR cfDNA fragments (black bars) of different sizes. Annealing sites for more than one forward primer (black arrows) and hydrolysis probe (blue bar: 6-FAM-labelled probe; green bar: HEX-labelled probe) only become available in OR cfDNA fragments exceeding 165 bp. Our assay also includes the co-amplification of a diploid locus (STAT6) to estimate absolute DNA concentration. A visualization of the raw data derived from the analysis of 1 ng of cfDNA can be observed in the right panel.

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