Table 1 Ratio between short (73–165 bp) and medium-sized (166–253 bp) OR fragments when using variable inputs from the same cfDNA sample in ddPCR reactions. Inputs below 5 ng generate reasonably similar ratios after analysing two assay replicates per sample. The short to medium-sized OR ratio nonetheless starts deflating for cfDNA inputs above 5 ng (see discussion). The ratio calculated from the 12.45 ng input is the only one that is significantly different (p < 0.001) from the other calculated ratios.
cfDNA INPUT (ng) | OR short/medium ratio | Poisson max ratio | Poisson min ratio |
|---|---|---|---|
0.30 | 3.70 | 4.20 | 3.10 |
0.42 | 3.66 | 4.12 | 3.19 |
0.72 | 4.00 | 4.43 | 3.57 |
1.66 | 3.67 | 3.92 | 3.42 |
3.80 | 3.72 | 4.08 | 3.38 |
4.70 | 4.03 | 4.21 | 3.85 |
6.25 | 3.22 | 3.34 | 3.09 |
12.45 | 2.47 | 2.54 | 3.39 |
