Figure 4

Phylogenetic analysis of the partial RdRp region of the duck picornaviruses and of representative viruses from Picornaviridae family. The picornavirus sequences detected in the Australian ducks from the current study tentatively belong to four different genera: Sicinivirus, Anativirus, Megrivirus and Aalivirus. The evolutionary history was inferred by using the Maximum Likelihood method based on the LG+G+I⁶⁶ model. The analysis involved 129 amino acid sequences. All positions containing gaps and missing data were eliminated. There were a total of 313 amino acid positions in the final dataset. The robustness of different nodes was assessed by bootstrap analysis using 1,000 replicates for amino acid alignments. The numbers at the nodes represent bootstrap values and only bootstrap values at or above 60% are shown. Each of the genus from Picornaviridae is shown with viruses from the duck samples in blue colour. Pacific black duck viruses are shown in black triangle, Chestnut teal viruses in brown square and Wood duck viruses in green circle. Supergroup 1 consists of viruses from genus Ailurivirus, Aphthovirus, Bopivirus, Cardiovirus, Cosavirus, Erbovirus, Hunnivirus, Malagasivirus, Mischivirus, Mosavirus, Mupivirus, Senecavirus, Teschovirus, Torchivirus and Tottorivirus. Supergroup 5 consists of viruses from genus Crahelivirus, Fipivirus, Gruhelivirus, Hepatovirus, Rohelivirus and Tremovirus. Supergroup 6 contains virus from the genus Harkavirus. Supergroup 7 consists of virus from the genus Ampivirus.