Figure 4
ALPP knock-down MDA-MB-231 cells cultured with an osteogenic cocktail (OC) do not show placental alkaline phosphatase (PLAP) expression, calcifications, and alkaline phosphatase (ALP) enzyme activity MDA-MB-231 cells were cultured for 6 weeks with OC including an ALPP siRNA or a negative control of OC alone (a) Western blot for PLAP after culture for 2 weeks. (b,c) Alizarin staining shows calcifications (c: magnification × 10 and × 20, scale bar 10 µl). (d,e) ALP staining shows ALP enzyme activity (e: magnification × 10 and × 20, scale bar 10 µm).
