Figure 5 | Scientific Reports

Figure 5

From: Osteogenic cocktail induces calcifications in human breast cancer cell line via placental alkaline phosphatase expression

Figure 5

Pharmacologically inhibited PI3K-Akt pathway, MDA-MB-231 cells cultured with an osteogenic cocktail (OC) do not show placental alkaline phosphatase (PLAP) expression and production of calcifications MDA-MB-231 cells were cultured for 6 weeks with vehicle, OC + vehicle, OC + LY294002, OC + Akt inhibitor X, and OC + AZD-8055. (a) Western blot for placental alkaline phosphatase (PLAP), c-Jun, and phospho-c-Jun after culture for 2 weeks. (b,c) Alizarin staining shows calcifications only with OC + vehicle (c: magnification × 10 and × 20, scale bar 10 µm).

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