Figure 4

Effects of PBI-4547 on glucose and FA metabolism in liver. (A) Relative gene expression levels of glucose and FA-related metabolism in liver from STD, HFD and HFD + PBI-4547 mice. Data are presented as geometric mean ± SEM (n ≥ 6 per group, one-way ANOVA with Dunnett’s multicomparison test vs HFD group). (B) 1H-NMR metabolic profiling in liver tissues. (C) Oxygen Consumption Rate (OCR) and Extracellular Acidifcation Rate (ECAR) in HepG2 hepatocytes. Measurements of basal respiration, maximal respiration, ATP production and proton leak in HepG2 hepatocytes treated or not with PBI-4547. (D) Oxidation rate of ¹⁴C-palmitate in HepG2 hepatocytes treated or not with PBI-4547. Data are presented as mean ± SEM (n = 3, Student t test Ctrl vs PBI-4547). See also Fig. S2.