Figure 4 | Scientific Reports

Figure 4

From: Three-dimensional bright-field microscopy with isotropic resolution based on multi-view acquisition and image fusion reconstruction

Figure 4

Three-dimensional imaging of a transgenic pEXP7:YC3.6 Arabidopsis thaliana seedling. Transverse (a) and lateral (b) sections of root tip (mature zone) acquired with bright-field multi-view reconstruction. Transverse (c) and lateral (d) sections acquired with bright-field reconstruction (grey) and LSFM (green). Maximum intensity projection of the LSFM stack, combined with the minimum intensity projection of the bright-field reconstruction (e). Detail of panel e, showing the outgrowing part of the root-hair epidermal cells (f). Three-dimensional rendering of the reconstructed bright-field volume, combined with LSFM. Scale bar: 100 µm.

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