Figure 5 | Scientific Reports

Figure 5

From: Three-dimensional bright-field microscopy with isotropic resolution based on multi-view acquisition and image fusion reconstruction

Figure 5

Sagittal slice of a transgenic Tg(kdrl:GFP) zebrafish (4 dpf) visualized with bright-field multi-view reconstruction (a). Details of the sample showing a portion of the head (b) and of the zebrafish notochord (c). Minimum intensity projection of the bright-field reconstruction (grey) overlapped with the maximum intensity projection of the LSFM data (green). The bright-field reconstruction shows the whole zebrafish anatomy while LSFM shows the labelled vasculature (d). Transverse section of the sample in the head (e) and tail (f), showing the bright-field reconstruction (grey) and LSFM (green). Scale bars: 100 µm.

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