Figure 5

stj and stol affect dendrite development. (A–C) Reconstructions of adult MN5 dendrite in control (A, n = 5) and following stj^RNAi (B, n = 4) and stol^RNAi (C, n = 6). (D–G) Morphometric parameters were analyzed. Following stj^RNAi as well as stol^RNAi total dendritic length (D, TDL, control: 6,655 ± 307 µm vs. stj^RNAi: 4,796 ± 329 µm, ***p = 2.37*10⁻⁴ and control vs. stol^RNAi 4,899 ± 833 µm, **p = 0.009; one-way ANOVA with Games–Howell post hoc test) as well as the number of dendritic branches (E, # branches, control: 4,241 ± 375 µm vs. stj^RNAi: 2,914 ± 586 µm, **p = 0.005 and control vs. stol^RNAi: 3,122 ± 700 µm, **p = 0.007, one-way ANOVA with LSD post hoc test) are significantly reduced. Other parameters like mean dendrite length (F, MDL, control: 1.57 ± 0.14 µm vs. stj^RNAi: 1.7 ± 0.2 µm, and control vs. stol^RNAi: 1.67 ± 0.11 µm; one-way ANOVA, p = 0.601) and mean path length (G, MPL, control: 9.02 ± 0.6 µm vs. stj^RNAi: 9.09 ± 0.87 µm, and control vs. stol^RNAi: 9.41 ± 0.74 µm; Kruskal Wallis ANOVA, p = 0.415) are not affected.