Figure 2
From: Development of a miniaturized 96-Transwell air–liquid interface human small airway epithelial model
Characterization and comparison of small airway epithelial cell ALI cultures between 24- and 96-Transwell formats. (a) Schematic of small airway epithelium and their respective cell types, cultured in a single Transwell of a Transwell-plate (b, c) Confocal images (XY 3D) of the four different cell types present in small airway epithelial cell culture, cultured at the air–liquid interface after four weeks under either 24-Transwell (b) or 96-Transwell (c) conditions. KRT5 for basal cells (red), AcTubulin for ciliated cells (blue), MUC5AC for goblet cells (green) and SCGB1A1 for club cells (yellow). Scale bar = 50 µm (d, e) Images shown are representative of a high intensity projection (XZ 3D) of either 24-well culture (d) or the HTS adapted 96-well culture (e). Scale bar = 25 µm. (f) Microscopic images of the glycoprotein staining in the small airway epithelial cells after 1 week and (g) after 4 weeks of air–liquid interface culture in 96-Transwell plates. Scale bar = 200 µm. (h) Gene expression analysis of different small airway epithelial markers MUC5AC (24: N = 3, n = 8; 96: N = 3, n = 7), MUC5B (24: N = 3, n = 8; 96: N = 3, n = 8); FOXJ1 (24: N = 3, n = 8; 96: N = 3, n = 7); KRT5 (24: N = 3, n = 8; 96: N = 3, n = 8).
