Figure 2

Sweat reduction by M-LNP. (a) Procedure of sweat assay on mouse footpads using the iodine/starch method. (b) Sweating area (%Area of black pixel fraction) on the right (R) and left (L) hind footpads of mouse groups treated with agents only on the right footpads by subcutaneous injection or skin-application. Groups: no treatment, PBS (0.8 mL kg⁻¹), pilocarpine HCl (2.5 mg kg⁻¹, negative control), glycopyrrolate bromide (0.25 mg kg⁻¹, positive control), BoNT/A (Meditoxin; 0.8 U kg⁻¹, positive control), myricetin (M; 0.8 mg kg⁻¹), Blank-LNP (0.8 mL kg⁻¹), and M-LNP (0.8 mg kg⁻¹). Data are expressed as the mean ± s.d. (standard deviation; n = 5; Student’s t-test; ns non-significant, *p < 0.05, **p < 0.01).