Figure 3
From: Reduction of focal sweating by lipid nanoparticle-delivered myricetin
Cell uptake of M-LNP. (a) The % amount of norepinephrine released from differentiated PC12 cells after treatments with BoNT/A (50 pM), myricetin (M; 20 μM), blank-LNP (LNP), and M-LNP (20 μM) with respect to that from non-treated cells. Data are expressed as the mean ± s.d. (b) Cumulative amount of myricetin released from the M-LNP matrix in the sink (10 vol% ethanol in PBS at pH 5.0 and 37 °C) and non-sink conditions (PBS at pH 5.0 and 37 °C). (c) Quantification of free fatty acids released from the M-LNP matrix lipolyzed by esterase and lipase at pH 7.0 and 37 °C. (d) Physicochemical characteristics of M-LNP and M-LNP deformed by lipolysis (M-dLNP) (PS particle size, ZP ζ-potential, EE efficient efficiency). Flow cytometry of (e) the adult human dermal fibroblasts (HDFa) and (f) PC12 cells incubated with the culture media containing NR, NR-LNP, and NR-dLNP. Graphs were obtained from histograms in Supplementary Fig. S4d using fitting regression procedures.
