Figure 5
From: Dodecin as carrier protein for immunizations and bioengineering applications
SDS-PAGE of the SpyT/-C and SnpT/-C reactions. Left: Reactions of mtDod Spy-/SnpT constructs with seACP-SpyC and/or mClover3-SnpC. Right: Inverse reactions of mtDod SpyC constructs with SpyT-seACP. In all reactions, bands of higher mass representing the conjugation products are observed. As mentioned above, the acidic loading buffer causes the appearance of double bands (mtDod SpyC constructs) and smearing bands (seACP-SpyC-H8) for some constructs.
