Table 1 Selection of mtDod constructs used for expression studies are divided into two groups: mtDod-peptides (constructs with only short peptides fused to mtDod) and mtDod-proteins (constructs with domains or entire proteins fused to mtDod). mtDod(WT): wild type mtDod. seACP: Saccharopolyspora erythraea ACP, gene chlB2. msfGFP: monomeric superfolder green fluorescent protein29. For a full description of constructs, see Supplementary Table S1. The constructs mtDod-GSG-Lys and mtDod-PAS-Met have been used as control; MtDod-GSG-Lys was used for its flexible linker, and mtDod-PAS-Met for its rigid linker. The PAS linker were based on sequences (slight alterations) shown in ref30.
From: Dodecin as carrier protein for immunizations and bioengineering applications
Construct name | Linker system | Molar mass/Da | Expression state# | |||
---|---|---|---|---|---|---|
mtDod-peptides | ||||||
mtDod(WT) | – | 7,497 | Soluble | |||
mtDod-GSG-Lys | GSG | 8,411 | Soluble | |||
mtDod-PAS-Met | PAS | 8,876 | Soluble | |||
mtDod-SpyT | PASG | 10,458 | Soluble | |||
SpyT-mtDod | GPAS | 10,215 | Soluble | |||
mtDod-PAS2-SpyT | PAS2G | 11,447 | Soluble | |||
SpyT-PAS2-mtDod | GPAS2 | 11,205 | Soluble | |||
SpyT-mtDod-SnpT | GPAS / PASG | 13,142 | Soluble | |||
mtDod-proteins | ||||||
mtDod-mmACP | PAS | 17,994 | Soluble | |||
mtDod-mmACP-H8 | PAS | 19,334 | Soluble | |||
mtDod-msfGFP-H8 | PAS | 36,388 | Soluble | |||
mtDod-SpyC-H8* | PAS | 22,413 | Inclusion body | |||
H8-SpyC-mtDod | PAS | 22,072 | Inclusion body | |||
mtDod-SZ1** | PAS | 14,232 | Inclusion body | |||
mtDod-SZ3** | PAS | 13,396 | Inclusion body | |||
mtDod-seACP*** | PAS | 19,966 | Inclusion body |
Linker details | ||||||
GSG: | GGGGSGGGG | PAS: | SPAAPAPASPAS | |||
PASG: | SPAAPAPASPASGGSG | GPAS: | GGSGSPAAPAPASPAS | |||
PAS2G: | SPAAPAPASPASPAPSAPAASPAAGGSG | GPAS2: | GGSGSPAAPAPASPASPAPSAPAASPAA |