Figure 2

Specificity of lick behavior is distinct from withdrawal behavior. (A) (Left) Schematic illustration of near-threshold intensities taken from psychometric curve, (right) comparison of hit rate of lick (blue) and reflex (grey) reports at near-threshold intensities, showing no significant difference, which suggests both behaviors are comparable in detection of optogenetic stimulation. (B) Comparison of false alarm rate, showing the lick behavior has a significantly higher amount of false alarms than the reflex behavior, which suggests higher-order decision making plays a role in the lick response, (C) sensitivity (d’), and response bias (criterion) between lick (blue) and reflex (grey) reports, showing only a significant difference between behavior sensitivity, which is due largely to the significant difference in false alarm rate. Green region indicates near-threshold intensities, All results are shown as mean ± SEM (n = 40), asterisks in a-c indicate significance at P < 0.025 using a two-sided Wilcoxon signed rank test, b was tested for multiple comparisons with Bonferroni correction. Data was plotted using MATLAB R2019a (https://www.mathworks.com). The figure was created using Adobe Illustrator CC 2019 (https://www.adobe.com).