Figure 3
From: Fabrication of fluorescent pH-responsive protein–textile composites
Genetic engineering and expression of responsive fluorescent curli fibers. (a) A scheme representing fusion of CsgA with a flexible linker and pHuji. (b) The fluorescence response of CsgA–pHuji under different pH conditions. (c) Circular dichroism spectra normalized for concentration of WT CsgA and CsgA–pHuji. (d) Normalized amount of dye bound to pellets of WT CsgA and CsgA–pHuji bacterial cultures via a Congo Red pull-down assay. (e) Optical microscope images of freshly purified WT CsgA and CsgA–pHuji hydrogels. (f) SEM images of WT CsgA and CsgA–pHuji producing bacterial cultures. (g) pH assays with concentrated pellets of CsgA–pHuji producing biofilms. (h) Mean steady state fluorescence response of CsgA–pHuji hydrogels to different pH buffers ranging from 3–11. The non-linear fit was performed using the Henderson–Hasselbalch equation (Eq. 4).
