Figure 2
Transfer of co-transfected DF-1 onto primary B cells results in the production of molecular clones rCu-1 and rvv. (A) Complete schematic representation of IBDV rescue. (B) DF-1 cells were co-transfected with plasmids that express segment A and B for each molecular clone. After 72 h, cellular material was (i) lysed by three freeze–thaw cycles, (ii) mechanically detached or (iii) enzymatically detached using trypsin–EDTA and transferred onto B cells (107 cells/ml) seeded in 75 cm2 flask. Cultures were harvested 48 h post-transfer and supernatants were titrated. Results are from three independent experiments each with two biological replicates.
