Figure 3
EC50’s of the anti-NP IgG1s reformatted from the 25 representative scFvs binding to the 6 representative recombinant NPs measured with ELISA. (A) The binding affinities as measured with ELISA (y-axis) for the anti-NP IgG1s binding to the NPs are plotted against the concentrations of the IgG1s (x-axis). The EC50’s of the binding curves were determined by fitting the binding curves with standard sigmoidal curve model with the software Prism, and are listed in Supplementary Table S2. (B) The heat map summarizes the specificities of the 25 anti-NP IgG1s (x-axis) in recognizing the 6 representative NPs (y-axis). The preparation of the IgG1s and the ELISA measurement of the IgG1-NP interactions are described in Methods. The heat map and the dendrogram were generated with the gplots package of the R software35.
