Figure 3
From: Pharmacological activation of the circadian component REV-ERB inhibits HIV-1 replication
Silencing Rev-erbs increases HIV-1 promoter activity. (a) TZM-bl cells were transduced with control or shRev-erbĪ± or shRev-erbĪ²-encoding lentiviral vectors that also expressed a GFP reporter, and fluorescent images of GFP were obtained 48Ā h later to confirm transduction. (b) Total RNA was extracted from control or Rev-erb silenced TZM-bl cells at 48Ā h post-transduction and Rev-erbĪ± or Rev-erbĪ² mRNA levels quantified by qRT-PCR (meanāĀ±āS.E.M., nā=ā3, unpaired t test). (c) Control or Rev-erb silenced TZM-bl cells were lysed 48Ā h post-transduction and HIV-1 promoter activity measured by quantifying luciferase activity (meanāĀ±āS.E.M., nā=ā3, unpaired t test).
