Figure 4
Analysis of the electrophysiological properties of HEK293 cells in KCNQ2 homomeric and heteromeric S247L channels. (A) (a) Representative current traces of KCNQ2 WT (2 μg) (n = 20), S247L (2 μg) (n = 10), and KCNQ2 WT + S247L (1 μg:1 μg) (n = 10). (b) Current density versus membrane potential (from − 80 to 40 mV) for KCNQ2 WT, S247L, and KCNQ2 WT + S247L. The current density in homomeric transfected variants demonstrated the lowest conductance–current curve in homomeric S247L. The conductance–current curves for heteromeric KCNQ2 WT + S247L exhibited small increases and closely resembled the curve for homomeric S247L. *p < 0.05 for KCNQ2 WT versus S247L; + p < 0.05 for KCNQ2 WT versus KCNQ2 WT + S247L. (c) Normalized currents (G/Gmax) versus voltage (from − 80 to 40 mV) for KCNQ2 WT, S247L, and KCNQ2 WT + S247L Cells transfected with homomeric S247L exhibited lower currents than did cells with the WT. (B) (a) Representative current traces of KCNQ2 WT + KCNQ3 WT (1 μg + 1 μg) (n = 10) and KCNQ2 WT + S247L + KCNQ3 (0.5 μg + 0.5 μg + 1 μg) (n = 10) are shown. (b) Current density versus membrane potential (from − 80 to 40 mV) for KCNQ2 WT + KCNQ3 WT and KCNQ2 WT + S247L + KCNQ3. The conductance–current curve (from − 10 to 40 mV) in KCNQ2 WT + S247L + KCNQ3 differed from that of KCNQ2 WT + KCNQ3 WT cells (p < 0.05). *p < 0.05. (c) Normalized currents versus voltage in two conditions for KCNQ2 WT + KCNQ3 WT and KCNQ2 WT + S247L + KCNQ3. *p < 0.05.
