Figure 5
Analysis of the electrophysiological properties of HEK293 cells in KCNQ2 homomeric and heteromeric S247W channels. (A) (a) Representative current traces of KCNQ2 WT (2 μg) (n = 20), S247W (2 μg) (n = 10), and KCNQ2 WT + S247W (1 μg:1 μg) (n = 10). (b) Current density versus membrane potential (from − 80 to 40 mV) for KCNQ2 WT, S247W, and KCNQ2 WT + S247W. The current density in homomeric transfected variants demonstrated the lowest conductance–current curve in homomeric S247W from − 30 to 40 mV, and the conductance–current curve for KCNQ2 WT + S247W exhibited small increases and was almost identical to the curve for S247W after the addition of KCNQ2 WT. *p < 0.05 for KCNQ2 WT versus S247W; + p < 0.05 for KCNQ2 WT versus KCNQ2 WT + S247W. (c) Normalized currents (G/Gmax) versus voltage (from − 80 to 40 mV) for KCNQ2 WT, S247W, and KCNQ2 WT + S247W. The cells transfected with homomeric S247X exhibited lower currents than did cells with the wild type. *p < 0.05 for KCNQ2 WT versus S247W. (B) (a) Representative current traces of KCNQ2 WT + KCNQ3 WT (1 μg + 1 μg) (n = 10) and KCNQ2 WT + S247W + KCNQ3 (0.5 μg + 0.5 μg + 1 μg) (n = 10). (b) Current density versus membrane potential (from − 80 to 40 mV) for KCNQ2 WT + KCNQ3 WT and KCNQ2 WT + S247W + KCNQ3. The conductance–current curve (from − 10 to 40 mV) in KCNQ2 WT + S247W + KCNQ3 differed that of the KCNQ2 WT + KCNQ3 WT cells (p < 0.05). *p < 0.05. (c) Normalized current versus voltage for KCNQ2 WT + KCNQ3 WT and KCNQ2 WT + S247W + KCNQ3. *p < 0.05.
