Figure 7

Western blotting demonstrated the protein expression in S247X, S247L, and S247W. (a) In contrast to the KCNQ2 WT, KCNQ2 protein cell-membrane expression was not present in S247X (arrow). (b) Immunostaining with the anti-KCNQ2 antibody demonstrated the HEK293 cell-membrane expression of S247L, S247W, and S247X variants. Protein expressions were lower in S247L and S247W.