Figure 3

Dosing time-dependent difference in L-OHP-induced myelotoxicity in mice. (a) The decrease in the number of viable BMCs in mice at 72 h after injecting L-OHP (20 mg/kg, i.v.) or saline at ZT6 and ZT18. Cell viability was assessed by staining with calcein-AM on FACS. Values are the mean with S.D. (n = 4). **P < 0.01, significant difference between the two groups (F_3,12 = 9.361, P < 0.001, ANOVA with Tukey–Kramer’s post-hoc test). (b) The survival rate of undifferentiated (left) and differentiated (right) BMCs after injecting L-OHP (20 mg/kg, i.v.) or saline at ZT6 and ZT18. Lineage-positive and -negative BMCs were separated by FACS as undifferentiated and differentiated cells, respectively. Values are the mean with S.D. (n = 8). *P < 0.05, significant difference between the two groups (t₁₄ = 3.045, P = 0.012, unpaired t-test, two-sided). (c) Temporal expression profiles of MRP4 in undifferentiated and differentiated cells prepared from bone marrow of mice. Values are the mean with S.D. (n = 6). *P < 0.05, significant difference between the two groups (F_3,20 = 14.892, P < 0.001, ANOVA with Tukey–Kramer’s post-hoc test). Full-size images of western blotting are presented in Supplementary Fig. 6.